Ascospore isolation and genetic analysis in yeast without micromanipulator

Abstract

A new method of isolation of yeast spore population for genetic analysis has been developed. The method is based on digestion of ascus wall with the snail crop juice and on selective elimination of vegetative cells by treatment with 33.6% ethyl alcohol. Use of the visible selector genes for detection of the monospore clones makes the method more suitable. Application of the new method to genetic analysis of ''roughness"' in colony morphology proved the monogenic inheritance of this character.