Paradoxical effects of antioxidant action in the photohaemolisis system

Abstract

The influence of Mexidol (solution for intravenous administration, “PHARMASOFT Ltd”.) in its therapeutic (0,24–3,9 μg/мл) and more high (15,6 μg/мл) concentrations on photo induced lysis (with Radachlorin, “RADAPHARMA Ltd”, as a photo sensitizer and a monochrome diode 653 nm as a light source) of donor RBC was under study. Strong acceleration a photo induced haemolysis was found in Mexidol presence evidencing the preparation prooxidant action. The grade of the photo induced haemolysis acceleration was rising with the increase of Mexidol concentration up to 3,9 μg/мл. High Mexidol concentration (15,6 μg/мл) still remaining prooxidative were less effective than some of therapeutic concentrations. The spectral analysis showed that therapeutic mexidol concentrations (0,24–3,9 μg/мл) didn’t change absorption maximum of Radachlorin in its “red” maximum. High Mexidol concentration (15,6 μg/мл) caused a hypochromic shift of the “red peak”, and its decrease that is in agreement with our data on photo induced haemolysis. Possible mechanisms of Mexidol prooxidant activity in photoinduced haemolisis system are discussed. Refs 12. Fig. 1. Tabl. 2.