Chromatographic quantitation by absolute calibration using an additional standard

Abstract

Precision of quantitative chromatographic analysis using absolute calibration is determined by the reproducibility of absolute areas (heights) of analyte peaks. Their dispersion, mainly associated with losses on samples during injection, lead to an insufficient accuracy of quantitation. Solving this problem implies using an additional standard for absolute calibration. The proposed version is based on introducing of an additional standard to calibration and target samples and on the replacement of absolute peak areas with relative values, namely S(analyte) / S(additional standard). It is noteworthy there are no restrictions on the chemical origin of the additional standard. In this case, the mode proposed allows increasing precision (reduce random errors) of results, as well as reducing the number of replicates to achieve a required accuracy. Furthermore, there is no significant complication of the analytical procedure as a whole. Refs 10. Figs 3. Tables 3.