The study of the effect ofseveral agents which denaturate DNA on giant chromosomes from salivary glands of some Chironomides

Abstract

The effect of several agents on chromosomes obtained from salivary glands of Chironomus has been studied by the method of luminescent analysis. It has been shown that the treatment with HC1, acetic acid, formamide and high temperature changes the spectrum of luminescence of the giant chromosomes comparing to the control. In case of treatment with HC1 it has been found that as a result of denaturation the coefficient a have incomplete restoration comparing to the original value. The agents studied are ordered in a row according to the level of their denaturing effect. The effect of NH₂-groups of chromosomal proteins on the type of fluorescence of chromosomes was not significant.