Method of closing the residual cavity with composite material “LitAr” in combination with an antibiotic for chronic pleural empyema and features of reparative histogenesis

Abstract

Our research examines the features of reparative histogenesis in liquidation of rigid residual cavity in chronic pleural empyema by means of a composite material. An experiment was conducted using 30 laboratory rats to model limited chronic pleural empyema (formed by implantation in the right half of the chest cavity a silicone bead with a 9mm diameter) and determine the use of the composite material “LitAr” in combination with an antibiotic to eliminate a rigid residual cavity in chronic pleural empyema. The cavity over the course of 20 days observation was infected with the introduction of 0.5 ml of overnight culture suspension of 105 CFU of Klebsiella pneumonia (strain GISCO № 278). To create a model of limited chronic empyema it took 38 days. Then the infected cavity was filled with composite material and the antibiotic cefazolin was administered intramuscularly in the dose of 20 mg/kg two times per day for 10 days. The area subjected to sealing composite material was then excised for subsequent morphological study. The data showed that the combination of composite “LitAr” antibiotic accelerated vasculogenesis, optimized cell proliferation and fibroblast cytodifferentiation differon. This resulted in the migration of a large number of differentiated fibroblasts to the composite material. These formed on the site of the liquidated cavity as loose irregular connective tissue. Refs 12. Figs 4.