Scientific adviser's report  on the thesis of Shaban Ali «A study of the aggregation status of the RRM2 domain of TDP-43 protein under oxidative stress by NMR diffusion measurements», presented Bachelor's Degree in Applied Mathematics and Physics.  Ali Shaban studies the processes of destabilizing the structure and aggregation of proteins under conditions of oxidative stress on the example of the RRM2 domain of the TDP-43 protein by NMR diffusometry and temperature denaturation of proteins with simultaneous detection of NMR spectra. As one of the best senior students of St. Petersburg State University, he repeatedly received an advanced academic scholarship. A. Shaban since 2014 participates in the scientific work of the biomolecular NMR laboratory of SPbSU, making a significant contribution to the scientific results obtained at the intersection of fields of physics, chemistry and biology. A. Shaban is certainly a talented student, who has a competent scientific approach. He studied the operation and performed experiments on modern equipment of the Resource Center "Magnetic Resonance Methods and Research" of the SPbSU Research Park. The diversity of research areas, which the applicant was familiar with, allowed him to become erudite not only in the subject of final qualifying work, but also in areas adjacent to it.  The declared topic of the final work is formulated fully and accurately. The text has a clear and harmonious structure and reveals important aspects of biophysical processes, such as the aggregation and destabilization of the structure of proteins as a result of the formation of intermolecular disulfide bonds that occur under conditions of oxidative stress. Protein TDP-43 unites a class of neurodegenerative diseases, such as amyotrophic lateral sclerosis and frontotemporal lobar degeneration. The molecular mechanisms of the aberrant behavior of proteins considered in the present work are characterized in modern science only by crude methods that do not provide enough evidence for constructing consistent models. Thus, the urgency of the work is unquestionable, since it is caused by the need to understand at the molecular level the processes that occur in living organisms, what only could be achieved by the application of cutting edge experimental approaches.  The aim of this work is to study the aggregation of RRM2 and its structural destabilization under oxidative stress conditions. To achieve the goal, the research objectives have been successfully formulated and completely solved. The results were obtained in the course of elaborate complex experiments on the measurement of the diffusion coefficients and the parameters of the temperature denaturation of protein molecules. A. Shaban prepared protein samples under oxidative stress conditions, which was simulated by hydrogen peroxide treatment. Reduction conditions were simulated by treatment with dithiothreitol. Analysis of the diffusion coefficient values ​​showed that the aggregate RRM2 particles formed under oxidizing conditions have a molecular weight more than 10 times that of the monomeric RRM2 molecules. This fact proves the involvement of oxidative stress in the formation of inclusions bodies, which are the sign of TDP-43 proteinopaties. Experiments on temperature denaturation, in turn, confirmed the unfolded nature of protein aggregates. The set of experimental facts obtained allowed the more deep understanding of the general processes occurring with proteins under conditions of oxidative stress.  A. Shaban convincingly demonstrated the ability to solve scientific problems arising during the course of research. He conducted a thorough analysis of the literature, described the theory of experiments, planned and conducted measurements, analyzed the results and summarized them in the text of the graduation qualifying work. In addition to the main research, he has developed a set of procedures for approximating one-dimensional and two-dimensional NMR spectra, which was published on the Matlab Central website. Such a software package is necessary in the work of a huge number of researchers around the world.  Graduation qualification work is a complete research carried out by the applicant independently at a high scientific level. The scientific results are given in the work allow to qualify them as a significant contribution to the study of protein systems under oxidative stress conditions. The results obtained by A. Shaban are reliable, the conclusions and conclusions are justified.  Ali Shaban has high qualification and he can be awarded the Bachelor's Degree in Applied Mathematics and Physics.  Scientific advisor:  Associate Professor, Ph.D.  Andrei V. Komolkin